Azotobacter and Pseudomonas Population in Rhizosphere and Yield of Choy Sum following Chemical Fertilizer and Biofertilizer application
Abstract
Inoculation of beneficial microbes is a way to improve the population of rhizobacteria that has a role in nutrient cycle and hence reduce the level of chemical fertilizer. A pot experiment was performed to evaluate the effect of and consortia biofertilizer combined with various dose of NPK compound fertilizer on the population of nitrogen-fixing Azotobacter sp. and phosphate solubilizing Pseudomonas sp. in rhizosphere and yield of choy sum (Brassica juncea L.). The experimental was setup in completely randomized block design consisted of ten combinations of various biofertilizer and inorganic fertilizer dose; and three replications. The results showed that Biofertilizer inoculation with or without chemical fertilizer did not improve the population of Azotobacter and Pseudomonas in the rhizosphere but increase the shoot weight of choy sum. Higher shoot weight was showed by crops with ¾ dose of NPK combined with recommended or reduced dose of fertilizer; and recommended dose of NPK and biofertilizer. This experiment verified that the used of consortia biofertilizer composed of Nitrogen-fixing bacteria and phosphate-solubilizing microbes reduced 50-75% dose of NPK.
Keywords
Full Text:
PDFReferences
S. Sivasakthi, P. Saranraj and P. Sivasakthivelan. “Biological nitrogen fixation by Azotobacter sp.—A review”. Indo Asian Journal of Multidisciplinary Research. vol. 3, pp. 1274–1284. 2017.
S.B. Sharma, R.S. Sayyed, M.H. Trivedi, and T.A. Gobi, “Phosphate solubilizing microbes: sustainable approach for managing phosphorus deficiency in agricultural soils”, Springerplus. vol. 13, no. 2, pp. 587, 2013.
A. Saeid, E. Prochownik, and J. Dobrowolska-Iwanek, “Phosphorus solubilization by Bacillus Species”, Molecules. vol. 23, no.11, pp. 2897, 2018.
N.N. Danapriatna, N. N “Penjaringan Azotobacter sp. dan Azospirillum sp dari ekosistem lahan sawah sebagai sumber isolat pupuk hayati penambat nitrogen”. Jurnal Agrotek Indonesia vol.1, no.2, pp.115-122, 2016.
S. Mahato, and A. Kafle, “Comparative study of Azotobacter with or without other fertilizers on growth and yield of wheat in Western hills of Nepal”, Annals of Agrarian Science. vol. 16, no.3, pp. 250–256, 2018.
S. Sankaralingam, S. Eswaran, B. Balakan, M. Sundaram, and T. Shanka, “Screening and growth characterization of phosphate solubilizing bacterium Pseudomonas Aeruginosa”. Advances in Environmental Biology, vol.8, no.13, pp. 673-680, 2014.
R. Hindersah, M.R. Setiawati, P. Asmiran, and B.N. Fitriatin, “Formulation of Bacillus and Azotobacter consortia in liquid cultures: Preliminary research on microbes-coated urea”. International Journal of Agricultural System, vol.8, no.1, pp. 1-10, 2020.
A. Costacurta, and J. Vanderleyden. “Synthesis of phytohormones by plant-associated bacteria”. Critical Review on Microbiology, vol. 21, no. 1. pp. 1-18, 1995.
S Pons. S. Fournier, C. Chervin, G. Bécard, S. Rochange, F. Dit, et al, “Phytohormone production by the arbuscular mycorrhizal fungus Rhizophagus irregularis”. PLoS ONE, vol. 15, no, 10, e0240886, 2020.
E. J. Rubio, M.S. Montecchia, Tosi, M. F.D. Cassán, A. Perticari, and O.S. Correa. “Genotypic characterization of Azotobacteria isolated from Argentinean soils and plant-growth-promoting traits of selected strains with prospects for biofertilizer production”. The Scientific World Journal. 2013.
A. Iqbal and S. Hasnain. “Auxin producing Pseudomonas Strains: Biological candidates to modulate the growth of Triticum aestivum beneficially”. American Journal of Plant Sciences, vol. 4, no. 9, pp.1693-1700, 2013.
H. Naseem. A. Muhammad, A.S. Muhammad, and N. Khan, “Exopolysaccharides producing rhizobacteria and their role in plant growth and drought tolerance”. Review Journal on Basic Microbiology. Vol. 58, no. 12, pp.1009-1022, 2018.
Y. Shabatai, “Production of exopolysaccharides by Acinetobacter strains in a controlled fed-batch fermentation process using soap stock oil (SSO) as carbon source”. International Journal of Biologi Macromolecule, vol. 12, no. 2, pp. 145-152, 1997.
A.M. Abou zied, E.H. Abd El-Zaher, H.A.Elsharquawy and T. Hammad, “Exopolysaccharides Production and Characterization from Marine-Derived Penicillium commune KP942881.1 with Some Medical Potential Applications”. Egyptian Journal of Botany, vol. 57, pp. 17-30. 2007.
A. Rahimi, S.S. Moghaddam, M. Ghiyasi, and S. Heydarzadeh, ”The influence of chemical, organic and biological fertilizers on agrobiological and antioxidant properties of syrian cephalaria (Cephalaria syriaca L.)”. Agriculture, vol. 9, 122
T. Biswas, and S.C Kole. “Soil organic matter and microbial role in plant productivity and soil fertility”. In: Adhya, T., B. Mishra, K. Annapurna, D. Verma, D. and U. Kumar. (eds) Advances in Soil Microbiology: Recent Trends and Future Prospects Microorganisms for Sustainability. Vol 4. pp 219–238. Springer, Singapore. 2017. [online] https://doi.org/10.1007/978-981-10-7380-9_10
D.B. Zandonadi, M.P. Santos, L. Medici and J. Silva “Action of organic matter and its fractions on vegetables physiology. Horticultura Brasileira, vol. 32, no, 1, pp.14-20, 2014.
A. Ben-David and C.E. Davidson. “Estimation method for serial dilution experiments”. Journal of Microbiological Methods, vol. 107, pp. 214-221, 2014.
W.M. Pandeirot, N.N. Wanta dan B.A. Pinaria. “Populasi larva Plutella xylostella Linn. pada tanaman kubis di kelurahan Paslaten Kecamatan Tomohon Timur Kota Tomohon”. Cocos. 6(10). 11 hal. 2015. [online] https://ejournal.unsrat.ac.id/v3/index.php/cocos/article/view/8229. [Diakses tanggal 11 Desember 2022].
J.C. Pommerville “Fundamental of Microbiology”. Massachusetts: Jones & Bartlett Learning, Burlington, 2021.
L. Dong, Y. Li, J. Xu, J. Yang, G. Wei, L. Shen, W. Ding and S. Chen. “Biofertilizers regulate the soil microbial community and enhance Panax ginseng yields”. China Medicine. vol. 14, no.20, 2019.
J. Kihara, M. Bekunda, V. Chimonyo, A. Kimaro, B. Kotu, S. Lyimo, and W. Mhango. “Management of soil fertility through application of fertilizers” in Hoeschle-Zeledon, I., J. Odhong, and M. Bekunda (eds). Sustainable Agricultural Intensification. Chapter 4, pp 48-61. CAB International. Wellingford, UK. 2022.
DOI: http://dx.doi.org/10.30598/ajibt.v12i1.1774
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.