Penggunaan Adenin Sulfat Pada Perbanyakan Mikro Talas Jepang

W Hattu, Dj. F Parera, Simos H.T Raharjo

Abstract


Taro is a staple food source in several regions of Indonesia. One type of this crop, namely Japanese taro or satoimo [Colocasia esculenta (L.) Schott var. antiquorum], is widely consumed in Japan so that there are export opportunities into the country. To overcome the problem of lack of propagules in the cultivation of taro, quality seed production can be done with micro propagation. This study aimed to obtain MS tissue culture media with the right concentration of adenine sulfate for Japanese taro micro propagation. The study was designed as a single factor experiment with a Completely Randomized Design, with an experimental factors of adenine sulfate concentration consisting of 5 levels, namely: A0 (control, 0 mg / l), A1 (10 mg / l), A2 (60 mg / l ), A3 (110 mg / l), A4 (160 mg / l), and with 5 replications. The use of MS media with BAP 0.5 mg /l + adenine sulfate 160 mg/l gave the best results for shoot multiplication with an average number of shoots produced 21.40 per culture bottle, at 12 weeks after culturing. The success percentage of plantlet acclimatization was 86%, and tissue cultur propagules subsequently was able to grow normally under ex vitro conditions.


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References


Hussain, Z. dan R.K. Tyagi, 2006. In vitro corm induction and genetic stability of regenerated plants in taro [Colocasia esculenta (L.) Schott]. Indian . Biotech.5:535-542

Lingga P., B. Sarwono, F. Rahardi, P.C. Rahardja, J.J. Afriastini, R. Wudianto, W.H. Apriadji. 1990. Bertanam ubi-ubian. PT. Penebar Swadaya. Jakarta.

SEAMEO. 2013.; Talas Jepang (Satoimo) Tissue Culture-Service Laboratory SEAMEO BIOTROP, Bogor, Indonesia.

Pierik, R. L. M., 1987. In Vitro Culture of Hinger Plant. Martinus NijhoftPublisher. Netherlands.

Wetherell, D. F. 1982. Introduction to "in vitro" propagation. Avery Publishing Group Inc., Wayne, New Jersey, 16 pages.

Nandagopal, S. and B.D. Ranjitha Kumari.2006. Adenine sulphate induced high frequency shoot organogenesis in callus and in vitro flowering of Cichorium intybus L. cv. Focus - a potent medicinal plant. Acta Agric. Slovenica 87(2):415-425.

Damayanti, D., Sudarsono, I. Mariska dan M. Herman. Regenerasi pepaya melalui kultur in vitro. Jurnal AgroBiogen 3(2):49-54.

Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bio assays for tobacco tissue cultures. Physiol. Plant. 15:473-497.

Gaspersz, V., 1991. Metode Perancangan Percobaan. Armico. Bandung.

George, E. F. dan P. D. Sherrington. 1984. Plant Propagation by Tissue Culture. Eastern Press. England.

van Staden J., E. Zazimalova and E.F. George E.F. 2008. Plant growth regulators II: Cytokinins, their analogues and antagonist. In: George E.F., M. Hall and G.J. De Kleck GJ (eds.) Plant Propagation by Tissue Culture. Vol 1. The Background. Springer, The Netherlands, pp. 205-226.

Hoesen, D.S.H. 1990. Pemberian zat pengatur tumbuh adenin dan benzylaminopurine pada perbanyakan tanaman pisang (Musa spp.) kultivar Ambon, Raja Bulu dan Tanduk secara in vitro. Prosiding Seminar Biologi Dasar I. Puslitbang Biologi-LIPI. Bogor.

Wattimena GA. 1988. Zat Pengatur Tumbuh Tanaman. Pusat Antar Universitas Bioteknologi IPB Bekerjasama dengan Lembaga Swadaya Informasi IPB: Bogor.

Pazurkeiwicz-Kocot, K., A.J. Kita and A. Haduch. 2011. The effect of kinetin on the chlorophyll pigments content in leaves of Zea mays L. seedlings and accumulation of some metal ions. Inżynieria i Ochrona Środowiska 14(4):397-409.

Kristina, N.N., A. Husni, dan E. Gati. 1998. Propagasi tanaman adas secara in vitro. Warta Tumbuhan Obat Indonesia 4(1): 29-31.

Bhuiyan F.R. 2013. In vitro meristem culture and regeneration of three potato varieties of Bangladesh. Res. Biotechnol. 4:29-37.

Singh, A.K., K. M.K. Sharma, R. Chaudhary and R.S. Sengar. 2017. Effects of BAP and adenine sulphate on shoot regeneration from callus in potato (Solanum Tuberosum L.). Biotech Today:7(1): 49-51

Gardner F.P., R.B. Pearce dan Mitchell. 1991. Fisiologi Tanaman Budidaya. Herawati Susilo, Penerjemah; Jakarta: Penerbit Universitas Indonesia

Gaspar, T.H., C. Kevers, O. Faivre-Rampant, M. Cre`Vecoeur, C.L. Penel, H. Greppin and J. Demmes. 2003. Changing concepts in plant hormone action. In Vitro Cell. Dev. Biol. - Plant 39:85–106

van Staden J., E. Zazimalova and E.F. George E.F. 2008. Plant growth regulators II: Cytokinins, their analogues and antagonist. In: George E.F., M. Hall and G.J. De Kleck GJ (eds.) Plant Propagation by Tissue Culture. Vol 1. The Background. Springer, The Netherlands, pp. 205-226.

Husain, M.K., M. Anis and A. Shahzad. 2008. In vitro propagation of a multipurpose leguminous tree (Pterocarpus marsupium Roxb.) using nodal explants. Acta Physiol. Plant. 30:353-335

Nandagopal, S. and B.D. Ranjitha Kumari.2006. Adenine sulphate induced high frequency shoot organogenesis in callus and in vitro flowering of Cichorium intybus L. cv. Focus - a potent medicinal plant. Acta Agric. Slovenica 87(2):415-425.

Lavanya A.R., S. Muthukrishnan, V. Kumaresan, B.J.H Franklin and M.V. Rao M.V. 2012. In vitro micropropagation of Hildegardia populifolia (Roxb.) Schott & Endl., an endangered tree species from Eastern Ghats of Tamil Nadu, India. J. Agril. Tech. 8(5):1727-1744.

Khan, M.K., P. Misra, T. Sharma, P.K. Shukla and P.E. Ramteke. 2014. Effect of adenine sulphate on in vitro mass propagation of Stevia rebaudiana Bertoni. J. Med. Plant Res. 8(13):543-549.

Sharma, V., B. Kamal, N. Srivastava, A.K. Dobriyal and V.S. Jadon. 2016. In Vitro flower induction from shoots regenerated from cultured axillary buds of endangered medicinal herb Swertia chirayita H. Karst. Biotech Res International 2014:1-5

Du, H.M., D.M. Tang and D.F. Huang. 2006. ‘Fragrant taro’ [Colocasia esculenta (L.) Schott var. antiquorum]micropropagation using thidiazuron and benzylaminopurine. Hort. Sci. & Biotech. 81:(3)379–384.

Ko, C-Y., J-P Kung and R. Mc Donald. 2008. In vitro micropropagation of white dasheen (Colocassia esculenta). African J. of Biotech. 7(1):041-043.

Lalopua, J.R., R.E. Wattimena, A. Walsen dan S.H. Raharjo. 1989. Penelitian tanaman umbian pada Fakultas Pertanian Universitas Pattimura, p.95-122. Dalam: Risalah Seminar Pengembangan Tanaman Umbian. Ambon, 31 Oktober 1989, Fakultas Pertanian Universitas Pattimura - United State Agency for International Development.




DOI: http://dx.doi.org/10.30598/a.v7i2.763

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